cdnas for oct4 pou5f1 (Addgene inc)

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Addgene inc cdnas for oct4 pou5f1
Cdnas For Oct4 Pou5f1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdnas+for+oct4+pou5f1/POU5F1+(Plasmid+%23155834)/us10494611-171-11-20
Average 93 stars, based on 23 article reviews

cdnas for oct4 pou5f1 - by Bioz Stars, 2026-10

93/100 stars

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Transfection:

Article Title: Compositions comprising a mitofusin inhibitor for promoting cell reprogramming and a use thereof
Article Snippet: Human foreskin fibroblasts (hFFs; ATCC) were maintained in DMEM containing 10% FBS, 1% NEAA, 1 mM L-glutamine, and 0.1 mM β-mercaptoethanol. .. GP2-293 packaging cells were co-transfected with pMX vectors containing the human cDNAs for Oct4 (POU5F1), Sox2, Klf4, and c-Myc (OSKM, Addgene) and the VSV-G envelope vector using Lipofecamine 2000 transfection reagent (Invitrogen). .. Virus-containing supernatants were collected at 48 and 72 hours after transfection and concentrated via ultracentrifugation (Beckman Coulter) at 25,000 rpm for 90 minutes.

Article Title: Mitofusins deficiency elicits mitochondrial metabolic reprogramming to pluripotency
Article Snippet: Human foreskin fibroblasts (ATCC) were maintained in DMEM containing 10% fetal bovine serum, 1% NEAA, 1 mM L-glutamine, and 0.1 mM β -mercaptoethanol. .. Virus production and iPSC generation GP2-293 packaging cells were co-transfected with pMX vectors containing the human cDNAs for Oct4 (POU5F1), Sox2, Klf4, and c-Myc (OSKM, Addgene, Cambridge, MA, USA) and the VSV-G envelope vector using Lipofectamine 2000 transfection reagent (Invitrogen). .. Virus-containing supernatants were collected at 48 and 72 h after transfection and concentrated via ultracentrifugation (Beckman Coulter, Brea, CA, USA) at 25 000 r.p.m. for 90 min. To generate iPSCs, MEFs were seeded at 1 × 10 5 cells per well in 6-well plates the day before transduction and were subsequently transduced with concentrated virus at a multiplicity of infection of 1 in the presence of Polybrene (8 μ g/ml).

Article Title: Mitofusins deficiency elicits mitochondrial metabolic reprogramming to pluripotency
Article Snippet: Human foreskin fibroblasts (ATCC) were maintained in DMEM containing 10% fetal bovine serum, 1% NEAA, 1 mM L-glutamine, and 0.1 mM β -mercaptoethanol. .. GP2-293 packaging cells were co-transfected with pMX vectors containing the human cDNAs for Oct4 (POU5F1), Sox2, Klf4, and c-Myc (OSKM, Addgene, Cambridge, MA, USA) and the VSV-G envelope vector using Lipofectamine 2000 transfection reagent (Invitrogen). .. Virus-containing supernatants were collected at 48 and 72 h after transfection and concentrated via ultracentrifugation (Beckman Coulter, Brea, CA, USA) at 25 000 r.p.m. for 90 min. To generate iPSCs, MEFs were seeded at 1 × 10 5 cells per well in 6-well plates the day before transduction and were subsequently transduced with concentrated virus at a multiplicity of infection of 1 in the presence of Polybrene (8 μ g/ml).

Article Title: Mitofusins deficiency elicits mitochondrial metabolic reprogramming to pluripotency.
Article Snippet: .. GP2-293 packaging cells were co-transfected with pMX vectors containing the human cDNAs for Oct4 (POU5F1), Sox2, Klf4, and c-Myc (OSKM, Addgene, Cambridge, MA, USA) and the VSV-G envelope vector using Lipofectamine 2000 transfection reagent (Invitrogen). .. Viruscontaining supernatants were collected at 48 and 72 h after transfection and concentrated via ultracentrifugation (Beckman Coulter, Brea, CA, USA) at 25 000 r.p.m. for 90 min. To generate iPSCs, MEFs were seeded at 1 × 105 cells per well in 6-well plates the day before transduction and were subsequently transduced with concentrated virus at a multiplicity of infection of 1 in the presence of Polybrene (8 μg/ml).

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Plasmid Preparation:

Article Title: DNp73 improves generation efficiency of human induced pluripotent stem cells
Article Snippet: .. The pMXs vector containing the human cDNAs for OCT4 (POU5F1), SOX2, c-MYC (MYC) and KLF4, described before [ ], and were obtained from Addgene. ..

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cdnas for oct4 pou5f1 (Addgene inc)

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